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. Author manuscript; available in PMC: 2015 Apr 4.
Published in final edited form as: Biochim Biophys Acta. 2014 Jan 7;1841(4):505–513. doi: 10.1016/j.bbalip.2013.12.015

Fig. 1.

Fig. 1

In vitro, microsomal LPLAT assays testing for C.a. LPT1 complementing S.c. lpt1Δ. LPLAT activity was measured in microsomes from S.c. lpt1Δ strains transformed with pRS423GP/C.a. LPT1 (4 CUG–UCG) or pRS423GP/– (vector only) 10 μg microsomes were incubated with 50 μM [14C]oleoyl CoA (20,000 dpm/nmol), 50 μM of lysoPA, lysoPC, lysoPE, lysoPI or lysoPS, and 100 mM Tris–HCl, pH 7.4 in 100 μl for 4 min. at 37 °C. After stopping the reactions, lipids were extracted, resolved using TLC, and quantified by scintillation counting. Data represent means ± standard deviation (n = 3) *p < 0.01.