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. Author manuscript; available in PMC: 2015 Apr 4.
Published in final edited form as: Biochim Biophys Acta. 2014 Jan 7;1841(4):505–513. doi: 10.1016/j.bbalip.2013.12.015

Fig. 3.

Fig. 3

Generation of homozygous, C.a. LPT1 deletion strains OMY15, OMY20. C. albicans strain SN152 (29) was transformed with a PCR-generated construct containing C.a. LEU2 flanked by ~60 nucleotides of C.a. LPT1 sequence (A). Transformants were selected on SC–Leu media and genotyped by PCR using oligo pairs lp1, lp2 or lp1, le2 (B). Subsequently, LPT1/lpt1Δ::LEU2 strains were transformed with a similar PCR-generated construct containing C.a. HIS1 with PCR genotyping using a lp1, hi2 oligo pair.