| Reagent | Final concentration |
|
| |
| Sodium phosphate dibasic (0.5 m; pH 7.0–7.5) | 0.1 m |
| Sodium phosphate monobasic (0.5 m; pH 7.0–7.5) | 5 mm |
| MgCl2 · 6H2O (1 m) | 3 mm |
| Sodium deoxycholate | 1.5 mm |
| Octylphenoxypolyethoxyethanol (IGEPAL CA-630) | 3.0% |
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