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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Aug 21;34(10):2268–2275. doi: 10.1161/ATVBAHA.114.303345

Fig. 4. Treatment of Tg (hsp70:DKK-1-GFP) zebrafish embryos with IWR-1 recapitulated Ang-2 as a Wnt target gene.

Fig. 4

(A) Tg (hsp70l:Dkk1-GFP) embryos were heat-shocked at 48 hpf at 37°C for 1 hour. Axin-2, a well-recognized Wnt target gene, was used as a reference control. In Tg (hsp70l:Dkk1-GFP) embryos, both Axin-2 and Ang-2 mRNA expressions were down-regulated in response to heat-shock induction of DKK-1 (*p < 0.05 vs. control, n=4). DKK-1 did not significantly change the expression of VE-Cadherin suggesting DKK-1-induced down-regulation of Axin-2 and Ang-2 was not due to potential vascular toxicity by heat-shock induction of DKK-1. (B,C) IWR-1 also down-regulated both Axin-2 and Ang-2 mRNA in a dose-dependent manner in the Tg (kdrl:GFP) fish at 72hpf. Ang-2 expression was down-regulated to a greater extent over 48 hr treatment (starting at 24 hpf) compared to 24 hr treatment (starting at 48 hpf). (*p < 0.05 vs. control; #p < 0.05 for pair-wise comparison, n=4).