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. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Aug 21;34(10):2268–2275. doi: 10.1161/ATVBAHA.114.303345

Fig. 5 — (A) Vasculature of transgenic zebrafish Tg (kdrl:GFP) at 72hpf reveals subintestinal vessel (SIV), intersegmental vessel (ISV), dorsal longitudinal vein (DLAV), dorsal aorta (DA), and posterior cardinal vein (PCV). (B) Embryos injected with the control MO developed normal SIV at 72 hpf. Ang-2 ATG-MO injection (0.5 μM) impairs SIV formation. Co-injection of zAng-2 mRNA (25ng) with Ang-2 MO rescued SIV formation. (C) Quantification of SIV length was performed and there was a significant difference between control and injection with morpholino (*p < .001, n = 20). zAng-2 mRNA injection rescued the SIV formation (^#p < .001, n = 20). (D) Treatment with Wnt signaling inhibitor IWR-1 impaired SIV formation. Injection with zAng-2 mRNA (25ng) was able to partially rescue SIV formation. (E) Quantification of SIV length showed significant reduction after IWR-1 treatment (*p < .001, n = 20), while injection with zAng-2 mRNA significantly increased SIV length (^#p < .001, n = 20).

Fig. 5 — (A) Vasculature of transgenic zebrafish Tg (kdrl:GFP) at 72hpf reveals subintestinal vessel (SIV), intersegmental vessel (ISV), dorsal longitudinal vein (DLAV), dorsal aorta (DA), and posterior cardinal vein (PCV). (B) Embryos injected with the control MO developed normal SIV at 72 hpf. Ang-2 ATG-MO injection (0.5 μM) impairs SIV formation. Co-injection of zAng-2 mRNA (25ng) with Ang-2 MO rescued SIV formation. (C) Quantification of SIV length was performed and there was a significant difference between control and injection with morpholino (*p < .001, n = 20). zAng-2 mRNA injection rescued the SIV formation (^#p < .001, n = 20). (D) Treatment with Wnt signaling inhibitor IWR-1 impaired SIV formation. Injection with zAng-2 mRNA (25ng) was able to partially rescue SIV formation. (E) Quantification of SIV length showed significant reduction after IWR-1 treatment (*p < .001, n = 20), while injection with zAng-2 mRNA significantly increased SIV length (^#p < .001, n = 20).