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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Jul 24;1842(10):1475–1482. doi: 10.1016/j.bbalip.2014.07.009

Figure 2. Glycolytic response in CD4+ T cells.

Figure 2

a) CD4+ T cells were isolated from age matched WT or GPAT-1 KO mice and stimulated with CD3/CD28 antibodies in complete medium. Cells were harvested and plated onto poly-l lysine coated dishes and basal oxygen consumption rate was analyzed followed by timed injection of oligomycin, FCCP, and rotenone. Each point represents the mean +/− the SEM of five individual mice in triplicate on the plate. The data in a) was used to calculate glycolytic response: b)Basal; c) Coupled respiration following oligomycin treatment; d); Uncoupled respiration following FCCP treatment; e) Following rotenone treatment. Each bar represents the mean +/− SEM of five individual mice in triplicate (for inhibitor addition) or sextuplet (for basal readings), and averaged across three time points. *Significantly different (p<0.05). Significance was determined using two-tailed student’s T- test. N=5.

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