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(A) The reaction catalyzed by methionine adenosyltransferase. (B) Turnover of S/Se-Met analogs to the corresponding AdoMet analogs catalyzed by sMAT based upon RP-HPLC (average percent error ≤4%, see Table 2 and Fig. 4). Bars colored green (referring to branched L-alkyl-substituted analogs) denote analogs for which only trace turnover was observed with MATs studied to date [15]. As controls, no product formation was observed in the absence of sMAT, S/Se-Met analogs or ATP.
