Table 2.
Summary of sMAT reactions
| Analog | Analog name | Percentage Turn-Over |
Theoretical mass | Observed mass |
|---|---|---|---|---|
|
Methionine | 100 | 399.1445, 297.0896[a] | 399.1444, 298.0965[b] |
|
Se-Methionine | 100 | 447.0890, 345.0340[a] | 447.0895, 346.0416[b] |
|
(2S)-2-amino-4-(ethylsulfanyl)butanoic acid | 84 | 413.1602, 311.1052[a] | 413.1599, 312.1121[b] |
|
(2S)-2-amino-4-(ethylselanyl)butanoic acid | 76 | 461.1046, 359.0497[a] | 416.1040, 360.0565[b] |
|
(2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid | 36 | 321.0896[a] | 322.1051[b] |
|
(2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid | 28 | 369.0340[a] | 370.0411[b] |
|
(2S)-2-amino-4-(propa-1,2-dien-1-ylsulfanyl)butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-[(cyanomethyl)sulfanyl]butanoic acid | 68 | 322.0848[a] | 323.0919[b] |
|
(2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid | 12 | 370.0293[a] | 371.0371[b] |
|
(2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid | 27 | 425.1602, 323.1052[a] | 425.1609, 324.1129[b] |
|
(2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid | 30 | 371.0497[a] | 372.0568[b] |
|
(2S)-2-amino-4-(propylsulfanyl)butanoic acid | 44 | 427.1758, 325.1209[a] | 427.1759, 326.1281[b] |
|
(2S)-2-amino-4-(propylselanyl)butanoic acid | 66 | 475.1203, 373.0653[a] | 475.1200, 374.0726[b] |
|
(2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid | 53 | 427.1758, 325.1209[a] | 427.1762, 326.1282[b] |
|
(2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid | 40 | 373.0653[a] | 374.0743[b] |
|
(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid | 30 | 437.1602, 335.1052[a] | 437.1599, 336.1124[b] |
|
(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid | 10 | 383.0497[a] | 384.0566[b] |
|
(2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid | 27 | 439.1758, 337.1209[a] | 439.1754, 338.1275[b] |
|
(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid | 15 | 439.1758, 337.1209[a] | 439.1754, 338.1282[b] |
|
(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid | 46 | 385.0653[a] | 386.0730[b] |
|
(2S)-2-amino-4-(butylsulfanyl)butanoic acid | 27 | 441.1915, 339.1365[a] | 441.1966, 340.1477[b] |
|
(2S)-2-amino-4-(butylselanyl)butanoic acid | 70 | 489.1359, 387.0810[a] | 489.1358, 388.0882[b] |
|
(2S)-2-amino-4-[(2-methylpropyl)sulfanyl]butanoic acid | 57 | 441.1915, 339.1365[a] | 441.1918, 340.1439[b] |
|
(2S)-2-amino-4-[(3-aminopropyl)sulfanyl]butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-[(3-aminopropyl)selanyl]butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid | 14 | 451.1758 | 451.1756 |
|
(2S)-2-amino-4-[(2-azidoethyl)sulfanyl]butanoic acid | 50 | 454.1616, 352.1066[a] | 454.1615, 353.1140[b] |
|
(2S)-2-amino-4-[(3-methylbutyl)sulfanyl]butanoic acid | 36 | 455.2071, 353.1522[a] | 455.2123, 354.1634[b] |
|
(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid | 12 | 453.1915 | 453.2044 |
|
(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid | 44 | 399.0810[a] | 400.0878[b] |
|
(2S)-2-amino-4-(cyclohex-2-en-1-ylsulfanyl)butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-(cyclohex-2-en-1-ylselanyl)butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-[(3-azidopropyl)sulfanyl]butanoic acid | 60 | 468.1772, 366.1223[a] | 468.1772, 367.1296[b] |
|
(2S)-2-amino-4-(benzylsulfanyl)butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-(benzylselanyl)butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-{[(2E)-4-azidobut-2-en-1-yl]sulfanyl}butanoic acid | 28 | 480.1772, 378.1223[a] | 480.1768, 379.1292[b] |
|
(2S)-2-amino-4-{[(2E)-4-azidobut-2-en-1-yl]selanyl}butanoic acid | 24 | 426.0667[a] | 427.0687[b] |
|
(2S)-2-amino-4-{[(2E)-4-aminobut-2-en-1-yl]selanyl}butanoic acid | 38 | 400.0762[a] | 401.0830[b] |
|
(2S)-2-amino-4-{[(4-methylphenyl)methyl]sulfanyl}butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-{[(2E)-3-phenylprop-2-en-1-yl]sulfanyl}butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-{[(4-nitrophenyl)methyl]sulfanyl}butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-{[(4-nitrophenyl)methyl]selanyl}butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-[(2-{[(3S)-3-amino-3-carboxypropyl]sulfanyl}ethyl)sulfanyl]butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-[(3-{[(3S)-3-amino-3-carboxypropyl]sulfanyl}propyl)sulfanyl]butanoic acid | ND | NA | NA |
|
(2S)-2-amino-4-{[(2E)-4-{[(3S)-3-amino-3-carboxypropyl]sulfanyl}but-2-en-1-yl]sulfanyl}butanoic acid | ND | NA | NA |
[a]
theoretical mass of degraded 5’-alkyl-thio(seleno)-5’-deoxyadenosine (MSeA); observed mass of degraded 5’-alkyl thio(seleno)-5’-deoxyadenosine (MSeA)
[b]
[M+H]
NDnot detected
NAnot applicable.