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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Anesth Analg. 2014 Oct;119(4):939–946. doi: 10.1213/ANE.0000000000000380

Figure 5. Effects of isoflurane exposure on Beclin-1 in the cerebral cortex.

Figure 5

A) Representative Western blot of Beclin-1 levels in the cerebral cortex after a 6 h exposure to isoflurane (1.5%ISO) or preconditioning (PC) with 1.5% isoflurane before the same 6 h isoflurane exposure (PC+1.5%ISO). B) Quantitative analysis of Beclin-1, normalized to β-actin as percent of control, showed that 1.5% isoflurane for 6 hours, with (P=0.53) or without isoflurane PC (P=0.71), or PC compared to controls (P=0.57) did not significantly affect the levels of Beclin-1 in the cerebral cortex at postnatal day 7 (P7). Controls, n=10; 1.5%ISO, n=10; PC+1.5%ISO, n=11. Data are presented as box plots of the means with whiskers (min to max).