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. 2014 Sep 18;9(9):e107463. doi: 10.1371/journal.pone.0107463

Figure 5. Endostar inhibits TCF/LEF-dependent transcriptional activity in HUVECs.

Figure 5

(A) Inhibition of TCF/LEF-dependent transcriptional activity in HUVECs by Endostar. HUVECs were transfected with pGL3-OT (a TCF/LEF-responsive reporter) or pGL3-OF (a negative control) plasmids plus pRL-SV40 as internal control and treated with different concentrations of Endostar. After 24 h, the luciferase activities were measured using the dual-luciferase reporter assay system and normalized to Renilla luciferase activity. *P<0.05 versus the control group. (B) Endostar suppresses the mutant β-catenin-mediated increase in TCF/LEF-dependent transcriptional activity. The pcDNA3-S33Y-β-catenin (a constitutively stabilized mutant β-catenin) plasmid and the reporter plasmid were cotransfected into HUVECs. After 12 h, cells were incubated with different doses of Endostar and the luciferase activities were determined. #P<0.05 versus the empty vector group. *P<0.05 versus the S33Y-β-catenin group.