Figure 1. Sequence alignment of classical (OMTKY3), non-classical group 1 (CrSPI-1-D1), non-classical group 2 (LDTI) and GmSPI-2 Kazal-family serine proteinase inhibitors.

The alignment was calculated with ClustalW2 [46]. Cysteine residues are highlighted in yellow. Amino acids identical in all four proteins are marked with an asterisk (*), conservative substitutions with a colon (:), and semi-conservative substitutions with a period (.).