Table 1. The oligonucleotide primer sequences for real-time RT-PCR.
Name | Sequences | |
β-actin | sense | 5′- CCCTGGCTCCTAGCACCAT-3′ |
antisense | 5′- CCTGCTTGCTGATCCACATCT-3′ | |
TGF-β | sense | 5′- GCCTGAGTGGCTGTCTTTTGA-3′ |
antisense | 5′- GAAGCGAAAGCCCTGTATTCC-3′ | |
MCP-1 | sense | 5′- CTCAGCCAGATGCAGTTAATGC-3′ |
antisense | 5′- GACACCTGCTGCTGGTGATTC-3′ | |
PAI-1 | sense | 5′- GCCCTACCACGGCGAAACCC-3′ |
antisense | 5′- TGATGGCGGAGAGGGGCACA-3′ | |
gp91phox | sense | 5′- TGGTGATGTTAGTGGGAGC-3′ |
antisense | 5′-3′ CTTTCTTGCATCTGGGTCT | |
Nox-1 | sense | 5′- TGGACGAATTAGGCAAACCG-3′ |
antisense | 5′- TTGGGGTGGGCAGTAGCTAT-3′ | |
α-SMA | sense | 5′- ACGGCGGCTTCGTCTTCT-3′ |
antisense | 5′- CCAGCTGACTCCATGCCAAT-3′ | |
type 1 collagen | sense | 5′- TCACCTACAGCACGCTTG-3′ |
antisense | 5′- GGTCTGTTTCCAGGGTTG-3′ | |
E-cadherin | sense | 5′- GTGCCACCACCAAAGATA-3′ |
antisense | 5′- GGCTGAGACAACCCTAAT-3′ | |
FSP-1(S100a4) | sense | 5′- AGCACTTCCTCTCTCTTGGTC-3′ |
antisense | 5′- GCTCCTTGAGCTCTGTCTTGT-3′ |
TGF-β; transforming growth factor β, MCP-1; monocyte chemoattractant protein 1, PAI-1; plasminogen activator inhibitor 1, α-SMA; alpha smooth muscle actin, FSP-1; fibroblast-specific protein-1.