Table 2. Photobleaching rates of TMR obtained from fits to fluorescent decay curves (estimates±95% confidence intervals).
Laser power(MW/cm2) | Delay (sec) | k (sec−1) |
1.29 | 0 | 0.0079±0.000018 |
0.64 | 0 | 0.0020±0.0000014 |
1.29 | 0.2 | 0.0010±0.0000020 |
Unlike GFP, the single exponential model for simple photobleaching was sufficient to explain the TMR fluorescent decay.