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. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: Am J Transplant. 2014 Sep 12;14(10):2359–2366. doi: 10.1111/ajt.12908

Kinetics of cellular and humoral immune responses against self-Ags. (A) IFN-γ, and IL-4 ELISpot responses against Col-V, and Kα1T, in mice that underwent syngeneic LTx (Bl/6 to Bl/6) and were administered Abs to Kα1T and Col-V were analyzed on day 7 and day 15. Represented as mean spots per million cells of an average of three wells per mice with at least 5 mice in each group. (B) ELISA was used to determine serum Abs (on day 7,15, 22) against Col-V, Kα1T, Col-II or Col-I in mice that underwent syngeneic LTx (Bl/6 to Bl/6) and were administered Abs to Kα1T and Col-V. Represented in μg/mL with at least 5 mice in each group.

Kinetics of cellular and humoral immune responses against self-Ags. (A) IFN-γ, and IL-4 ELISpot responses against Col-V, and Kα1T, in mice that underwent syngeneic LTx (Bl/6 to Bl/6) and were administered Abs to Kα1T and Col-V were analyzed on day 7 and day 15. Represented as mean spots per million cells of an average of three wells per mice with at least 5 mice in each group. (B) ELISA was used to determine serum Abs (on day 7,15, 22) against Col-V, Kα1T, Col-II or Col-I in mice that underwent syngeneic LTx (Bl/6 to Bl/6) and were administered Abs to Kα1T and Col-V. Represented in μg/mL with at least 5 mice in each group.