Figure 4. The lprG mutant is impaired for macrophages entry.

(A) Resting BMMΦ from C57BL/6 mice were infected with wild-type (H37Rv), lprG mutant (ΔlprG), and ΔlprG complemented with lprG-Rv1410c (::lprG) at MOI of 1∶10 (Input). At 4 h post-infection cells were washed and intracellular bacteria (Output) were enumerated on agar. Bar graph shows average CFU counts ±SEM of three independent experiments performed in triplicate wells. (B–D) RAW 264.7 macrophages treated with and without 4 mg/ml mannan or 0.5 µg/ml anti-MMR pAb were infected with FITC-labeled bacteria at MOI of 1∶100 for 2 h. Cells were washed, mounted and visualized using a fluorescent microscope. Graphs show percentage of infected cells with no treatment (B), treated with mannan (C) and treated with anti-MMR pAb (D). Bars show average percentage ±SEM of two independent experiments performed in triplicates. ΔlprG and ::lprG were compared to H37Rv. *P<0.05.