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. 2014 Sep 19;9(9):e107732. doi: 10.1371/journal.pone.0107732

Figure 1. Expression of mT2R138, mT2R108 and α-Gustducin (Gust) mRNA in the mouse Gastrointestinal Tract.

Figure 1

mRNA levels were analyzed in the different regions of the GI tract with qRT-PCR and normalized to β-actin levels in each tissue (A–C). Relative quantities were determined using the comparative ΔΔCt method. Each cDNA sample was amplified in duplicate and all data are expressed as the mean ± S.E.M. T2R138 expression was very low in the stomach compared to the small and large intestine (A), whereas T2R108 is more abundant in the stomach compared to the intestine (B). α-Gustducin is more abundant in the stomach and colon compared to the other regions (C). D: Single bands of the predicted size were found for each primer, in all GI segments analyzed as well as in STC1 cells or the tongue, which served as positive controls. No signal was detected with any of the primer in 3T3 cells, which were used as negative control. A:antrum, C:corpus, D:duodenum, J: jejunum, I: ileum, PC: proximal colon, DC: distal colon, STC1: STC 1 cells, BA: β actin; CTR, control; * the tongue is shown as control for α-gustducin.