Table 2. Bacterial strains and plasmids used in this study.

Strains or plasmids	Descriptions	Reference or source
K. pneumoniae
CG43S3	CG43 Smr	[41]
AP001	CG43S3ΔiscR	This study
AP002	CG43S3iscR 3CA	This study
AP003	CG43S3Δfur	[42]
AP004	CG43S3Δfur-ΔiscR	This study
AP005	CG43S3Δfur-iscR 3CA	This study
AP006	CG43S3ΔlacZ	[23]
AP007	CG43S3ΔlacZ-ΔiscR	This study
AP008	CG43S3ΔlacZ-iscR 3CA	This study
AP009	CG43S3ΔlacZ-Δfur	This study
AP010	CG43S3ΔlacZ-Δfur-ΔryhB	This study
AP011	CG43S3ΔlacZ-Δfur-ΔiscR	This study
AP012	CG43S3ΔgalU	[35]
E. coli
DH5α	supE44ΔlacU169 (f80 lacZΔM15)15)169recA1 endA1 gyrA96 thi-1 relA1	[43]
BL21(DE3)	F- ompT hsdSB[rB−mB−]gal dcm [DE3]	New England Biolabs
S17-1 λ pir	hsdR recA pro RP4-2 [Tc::Mu; Km::Tn7] [λpir]	[37]
Plasmids
pKAS46	Apr Kmr, positive selection suicide vector, rpsL	[36]
yT&A	Apr, TA cloning vector	Yeastern
pACYC184	TcrCmr, low copy number cloning vector	New England Biolabs
pIscR	Cmr, 980-bp fragment containing an iscR allele cloned into pACYC184	This study
pIscR3CA	Cmr, 980-bp fragment containing C92A, C98A and C104A mutant allele of iscR cloned into pACYC184	This study
placZ15	Cmr, promoter selection vector, lacZ +	[23]
piscRZ15	Cmr, 312-bp fragment containing the region upstream of iscR cloned into placZ15	This study
pOrf12	Cmr, 500-bp fragment containing the region upstream of Klebsiella K2 cps orf1-orf2 cloned into placZ15	[23]
pOrf315	Cmr, 900-bp fragment containing the region upstream of Klebsiella K2 cps orf3-orf15 cloned into placZ15	[23]
pOrf1617	Cmr, 300-bp fragment containing the region upstream of Klebsiella K2 cps orf16-orf17 cloned into placZ15	[23]
pET30b-IscR	Kmr, 654-bp fragment encoding full-length IscR cloned into pET30b	This study
pET30b-IscR3CA	Kmr, 654-bp fragment encoding full-length C92A, C98A and C104A mutant allele of iscR cloned into pET30b	This study
piscR-pKAS46	AprKmr, 2.0 kb fragment containing iscR and its flanking regions cloned into pKAS46	This study
piscR3CA-pKAS46	AprKmr, 2.0 kb fragment containing full-length C92A, C98A and C104A mutant allele of iscR and its flanking regions cloned into pKAS46	This study