Skip to main content
. 2014 Sep 19;9(9):e107661. doi: 10.1371/journal.pone.0107661

Figure 1. VM capacity and expression of RhoA, ROCK1 and ROCK2 in HCC cell lines.

Figure 1

(A) Formation of tubular network structures on Matrigel by HUVEC and LO2 compared to HCC cell lines. Cells were seeded into Matrigel-coated 24-well plates and incubated at 37°C. Photographs were taken after 24 h, with original magnification of 40×, scale bars represent 500 µm. (B) Western blot was performed to confirm the protein expression of RhoA, ROCK1 and ROCK2 in HCC cell lines and LO2, using HUVEC as a positive control. (C) Relative densities (RD) are presented as mean ± S.E. (n = 3) of the fold change relative to the internal control. GAPDH was used as an internal control for protein loading. *P<0.05 and **P<0.01 vs. HUVEC and # P<0.05 and ## P<0.01 vs. MHCC97H.