Figure 4. The effect of ROCK inhibition on the acquisition of an EMT phenotype.

Western blot analysis showed that MHCC97H cells subjected to Matrigel with culture medium treated (C) or not treated (A) with Y27632 for 48 h regulated Vimentin and E-cadherin expression. GAPDH was used as a loading control. Quantification of EMT markers is shown in (B) and (D). The data are reported as mean ± S.E. (*P<0.05 compared to 0 h or the control, n = 3).