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. 2014 Sep 19;9(9):e107447. doi: 10.1371/journal.pone.0107447

Figure 6. Effects of DMSO on apoptosis of cultured mouse cortical astrocytes.

Figure 6

Astrocytes were incubated with various concentrations of DMSO for 24 h. (A) Representative micrographs showing TUNEL positive apoptotic astrocytes (cyan-blue). Cell nuclei counterstained with Hoechst 33342 (blue). (B) Quantitative analysis of astrocyte apoptosis. (C) Representative western blot bands showing expression levels of procaspase-3, cleaved caspase-3 and Bcl-2 in astrocytes. (D) The quantitative analysis showed that Bcl-2 and procaspase-3 expression levels were decreased, but cleaved caspase-3 expression level was increased in astrocytes treated with 1% or 5% DMSO. Data are shown as a mean ± SEM of five (for TUNEL) or four (for Western blot) independent experiments performed in triplicate. *P<0.05 and **P<0.01 versus control group.