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. 2014 Sep 19;9(9):e107913. doi: 10.1371/journal.pone.0107913

Figure 2. Enforced expression of HBx inhibits CYP2E1 gene expression in HepG2 cells.

Figure 2

Plasmid expressing HBx (pCMV-2B–FLAG-X) or a control plasmid (pGL3 empty vector) was transfected into HepG2 cells. (A) Overexpression of HBx significantly inhibited CYP2E1 mRNA compared to the non-transfected cells or pGL3 empty vector transfected cells by RT-PCR with GAPDH as an internal control. (B) Effect of ectopic overexpression of HBx on CYP2E1 mRNA level was determined by real time quantitative PCR in HepG2 cells. The values were represented as the mean standard deviation; *, P<0.001 vs. the control group or the empty vector group.