Table 1. Primers used for making CYP2E1 promoter luciferase reporter gene constructs.
Construct Name | Sense (5′→3′) | Antisense (5′→3′) |
CYP2E1-P-Luc | CATTGTCAGTTCTCACCTC | GGACACCAGCAGGAGGAAG |
CYP2E1-P1-Luc | CAATGACTTGCTTATGTGG | GGACACCAGCAGGAGGAAG |
CYP2E1-P2-Luc | CCACAAGTGATTTGGCTGG | GGACACCAGCAGGAGGAAG |
CYP2E1-P3-Luc | TGCCAGTTAGAAGACAGAATG | GGACACCAGCAGGAGGAAG |
CYP2E1-P4-Luc | CATAGAAGGTGGAAGAGGG | GGACACCAGCAGGAGGAAG |
CYP2E1-P5-Luc | CCGGGATCAACAAAGACAAG | GGACACCAGCAGGAGGAAG |
CYP2E1-P6-Luc | CTACAGCCAGAATATATACC | GGACACCAGCAGGAGGAAG |
CYP2E1-P7-Luc | CTGGGGGCTGCTCAGACAAACC | GGACACCAGCAGGAGGAAG |
CYP2E1-P8-Luc | TATGGGTTGGCAACATGTTCCT | GGACACCAGCAGGAGGAAG |
CYP2E1-P9-Luc | GTGCTAGCAACCAGGGTGTTGA | GGACACCAGCAGGAGGAAG |
CYP2E1-P10-Luc | CTGGGGGCCACCATTGCGGGAA | GGACACCAGCAGGAGGAAG |