Figure 1. Label retaining assay in murine salivary glands.

A) Label Retaining Assay. At 10 days after birth (P10), FVB mice were pulsed with 4 doses of EdU (100 mg/kg) or BrdU (30 mg/kg) 12 hours apart. Tissue was collected from 10-week old animals. B) LRCs from 3 mice were manually quantified per salivary gland compartment (acinar and ductal). Data are expressed as percentage from the total of cells in each individual compartment for both parotid and submandibular glands. C) Comparison of labeling efficiency between EdU and BrdU. Data are shown as percentage of LRCs per individual compartment. A 2-sided unpaired T-test was performed for analysis (n = 5 per group). D–E) Representative images of parotid and submandibular glands of BrdU-pulsed animals. LRCs in the acinar compartment (a) are shown with white arrowheads. LRCs in ductal compartment (d) are pointed with black arrows. Example of ductal compartment is delineated by dashed line and pointed with black arrowhead F–K) Representative fluorescent images of salivary glands from EdU-pulsed animals. EdU LRCs are shown in green and DAPI in blue.