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. 2014 Sep 19;9(9):e107893. doi: 10.1371/journal.pone.0107893

Figure 5. Differentiation of Salivary gland Spheres.

Figure 5

A–B) Amylase staining (red) of parotid-derived spheres at days 2–3 in culture. C–D) Confocal images at Z = 0.5 um and Z = 6 um of double staining for amylase (red) and EdU (green) at day 4. Areas in yellow dashed squares are shown in C’ and D’. White arrow points at an amylase-positive cell with traces of EdU. Glands were obtained from mice at 10 weeks of age. E–G) Double immunofluorescence staining for Amylase (red) and EdU (Green) of parotid gland of 10-week old mice. White arrowhead points at LRCs in the acinar compartment; yellow arrowhead points at LRCs in ductal structures.