Table 2.

Effect of fixation conditions on HER2 immunochemistry (IHC) score in human breast cancer xenografted tumors

Time to fixation	NCI-N87				SCH				SNU-16
	Fixation time				Fixation time				Fixation time
	24 h	5 days	7 days	10 days	24 h	5 days	7 days	10 days	24 h	5 days	7 days	10 days
0 h	3+	3+	3+	3+	2+a	2+a	2+a	1+/2+	1+	1+	1+	0/1+
6 h	3+	3+	3+	3+	2+	2+/3+	2+	2+	1+	1+	1+	0/1+
24 h	3+	3+	3+	3+	1+/2+	1+a	1+/2+	1+/2+	1+	0/1+	1+	0

HER2 IHC was performed by HercepTest

Tests were performed in duplicate for NCI-N87 and SNU-16, in triplicate or duplicate (a) for SCH. If the test results of duplicate samples were different, both values were indicated