Table 6.
Fixation time | Evaluation | Fixatives | |||
---|---|---|---|---|---|
10 % NBF | 10 % nonbuffered formalin | 20 % NBF | 20 % nonbuffered formalin | ||
24 h | HER2 IHC scorea | 3+ | 3+ | 3+ | 3+ |
Histological assessment/decrease in stainingb | – | – | – | – | |
10 days | HER2 IHC scorea | 3+ | 3+ | 3+ | 3+ |
Histological assessment/decrease in stainingb | – | – | – | – |
Tests and assessments were performed in duplicate
aHER2 IHC was performed by HercepTest
bHistological assessment on the decrease in staining was performed in terms of the following criteria and judged in a comprehensive manner: (a) autolysis, (b) decrease in positive cells, (c) decrease of total staining intensity, (d) decrease of total staining area
−, no change, ±, very slightly decreased; +, slightly decreased, ++, moderately decreased; +++, markedly decreased