Skip to main content
. 2014 Oct;105:67–71. doi: 10.1016/j.mimet.2014.07.012

Fig. 3.

Fig. 3

AceE interacting proteins. M. smegmatis containing pTIG-N::aceE grown in LB + 0.05% Tween 80 was induced with 100 ng/ml ATc for 24 h and complexes crosslinked with 0.4% formaldehyde. The Strep/His-tagged complexes were purified as described in the Materials and Methods section, crosslinks were reversed by heating at 95 °C for 20 min and the proteins separated by SDS-PAGE. A control of wild type cells yielded no complexes and is not shown.