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. Author manuscript; available in PMC: 2014 Sep 20.
Published in final edited form as: Biometals. 2011 Mar 22;24(5):875–890. doi: 10.1007/s10534-011-9441-6

Fig. 3.

Fig. 3

Rapid induction of zinc dysregulation in ZnPT-treated in human skin keratinocytes. a Time course of increase in intracellular free zinc induced by ZnPT (1 μM) as detected by Zinquin fluorescence with representative fluorescence microscopy pictures: panel I: control; panel II: ZnPT (5 min); panel III: ZnPT (60 min). Quantitative analysis was performed as specified in “Materials and methods” (mean + SD). b Dose–response analysis of ZnPT-induced changes in Zinquin-fluorescence (0.1–5 μM; 5 min exposure). c Total cellular zinc content as analyzed by ICP-MS after ZnPT (0.5 and 1 μM, 1 h) and ZnSO4 (50 μM, 1 h) exposure (mean + SD; n = 3)