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. Author manuscript; available in PMC: 2015 Sep 18.
Published in final edited form as: Mol Cell. 2014 Aug 14;55(6):818–828. doi: 10.1016/j.molcel.2014.07.007

Figure 5. The RSC chromatin remodelers are required to prevent CAG repeat expansions, facilitate SCR, and are recruited to the CAG repeat.

Figure 5

(A, B) Instability was measured as in Fig. 1A; *p<0.05 to wildtype, Fisher’s exact test. (C) Spontaneous SCR rates were measured and analyzed as in Fig. 4A, B. (D) MMS-induced SCR as in Fig. 4D. (E–F) Recruitment of TAP-tagged Rsc proteins to 0.6 kb from the CAG repeat was measured by ChIP in α-factor synchronized cells at time points after release into fresh media; qPCR was run as in Fig 2B. Background levels were determined by IgG pull-down in a CAG-155 strain containing no TAP tag; percent of input sample precipitated is shown with no normalization to another locus. Averages and standard errors (SEM) for 2–4 independent replicates for each time point are shown.