Figure 2.
(A) Transfection efficiency and (B) cellular uptake in HEK293 cells of χ/M-VLP, PEI/M-VLP and PLL/M-VLP with 1 × 109 M-VLP/well of a 12-well tissue culture plate using M-VLP density of 10 × 109 M-VLPs/ml. Normalized Uptake = (mean fluorescence of treated sample - mean fluorescence of untreated samples)/mean fluorescence of untreated samples. Error bars indicate standard deviation (n=3). (C) Expression of luciferase reporter gene in HEK293 cells by χ/M-VLP in comparison to MLV-A over three weeks. Cells were trypsinized, split and re-seeded ~10 times during this period. (*, p < 0.002; #, p < 0.0005).
