. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: J Hepatol. 2014 May 22;61(4):903–911. doi: 10.1016/j.jhep.2014.05.024

Table 3.

Fold changes in the expression level of genes involved in fatty acid oxidation and lipogenesis

	Primary Hepatocytes	Pair fed	Alcohol fed	Alcohol + 17-DMAG – 50 mg/kg BW
Oxidation	CPT1a	1.00 ± 0.11	0.75 ± 0.26	4.12 ± 1.74^c
	ACOX1	1.00 ± 0.04	0.32 ± 0.06^a	1.25 ± 0.43^c
	LCAD	1.01 ± 0.15	0.59 ± 0.34^a	2.18 ± 0.79^c
	MCAD	1.00 ± 0.09	0.35 ± 0.14^a	1.40 ± 0.58^c
Lipogenesis	SREBPF1	1.01 ± 0.13	2.45 ± 0.78^a	0.99 ± 0.35^c
	ACC1	1.01 ± 0.17	2.04 ± 0.39^a	0.78 ± 0.53^c
	FAS	1.01 ± 0.17	1.73 ± 0.36^a	0.92 ± 0.13^c
	SCD-1	1.00 ± 0.08	3.07 ± 1.03^a	0.71 ± 0.57^c

Total RNA was isolated from primary hepatocytes and subjected to quantitative RT-PCR for indicated genes. Expression levels were normalized to 18S ribosomal RNA and compared to untreated controls, which were set at 1.0.

p<0.05, pair fed vs alcohol fed

p<0.05 alcohol fed vs alcohol + 50 mg/ kg BW 17-DMAG

Nonparametric ANOVA followed by Kruskal-Wallis test. Values shown are mean ± SE, n=6.