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. 2014 Sep 14;13:216. doi: 10.1186/1476-4598-13-216

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© Xu et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MAPK/ERK and TGF-β pathway were involved in SNORD113-1 effect. A, B. The relative reporter expression in HepG2 transfected with p3.1-SNORD113-1 (A) or SNORD113-1 siRNA (B) determined using Cignal Finder 10 Cancer Pathway Reporter Assays. HepG2 cells transfected with p3.1-SNORD113-1 or SNORD113-1 siRNA were seeded onto 96-well plates containing luciferase reporters to 10 common cancer pathways along with FuGene HD transfection reagent. Luciferase activity of the cells was measured using the dual luciferase assay system. Relative firefly luciferase activity was calculated and normalized to the constitutively expressed Renilla luciferase. C, D. The effect of SNORD113-1 on MAPK/ERK pathway (C) or TGF-β pathway (D). The expression and phosphorylation of key molecules of the pathways were detected using immunoblotting. GAPDH was used as an internal control.