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. 2014 Sep 4;15(1):759. doi: 10.1186/1471-2164-15-759

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© Song et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Hemoglobin staining of embryos injected with rps24 MO using o-dianisidine and effectiveness of rps24 MO. (A-F) O-staining of rps24 MO embryos showed a drastic reduction in the number of hemoglobin-stained blood cells when rps24 is knockdown (A and D are controls, B and E are rps24 knockdown) and partially rescued phenotype by co-injection of p53 MO (C and F). (G-H) The sequence of rps24 MO is a compliment of 1–24 bp of rps24 cDNA. Embryos co-injected with 25 ng rps24: egfp DNA and 5 ng control MO produced green fluorescent protein (G), and the expression of green fluorescent fusion protein was inhibited by co-injection with 2 ng rps24 Mo (H). A, B, C are ventral view; D, E, F, G and H are lateral view.