Fig. 2.

a Activity of LY2835219 in colo-205 and MCF10A cells using a multiplexed assay measuring p-Rb serine 780 (green), cells in G1 (2 N DNA content, red) and total cell number (black). after 24-hour exposure LY2835219 shows a dose-dependent inhibition of p-Rb (ser780) and a corresponding sustained G1 arrest. b MV4-11 acute myelocytic leukemia (AML) cells treated with increasing concentrations of LY2835219 for 24 h and cell cycle activity profiled by flow cytometry. c cell cycle activity of LY2835219 in cells with and without functional Rb. LY2835219 gives a G1 cell cycle arrest in Rb-proficient MDA-MB-231 breast cancer cells, but not in Rb-deficient MDA-MB-468 breast cancer cells