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. Author manuscript; available in PMC: 2014 Sep 21.
Published in final edited form as: Nat Immunol. 2009 Feb 22;10(4):420–426. doi: 10.1038/ni.1708

Figure 1.

Figure 1

Phosphorylation of AID by PKA modulates CSR. (a) Flow cytometry of GFP and surface IgG1 on Aicda−/− splenic B cells stimulated with LPS plus IL-4 and left uninfected or infected with retroviral vector expressing GFP alone (pMIG) or AID(WT) or AID(S38A) and a GFP indicator, assessed 96 h after infection. Numbers in quadrants indicate percent cells in each; numbers in parentheses indicate IgG1+ cells in the GFP+ gate. Data are representative of at least five independent experiments. (b) GFP+ IgG1+ cells in the total GFP+ population of wild-type splenic B cells stimulated with LPS plus IL-4 and infected with retrovirus vector control (pMIG) or retrovirus expressing PKA-Cα. *, P < 0.01 (two-tailed Student’s t-test). Data are representative of four independent experiments (mean and s.d.).