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. 2014 Sep 21;20:1366–1373.

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Copyright © 2014 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Dual RNAscope in situ hybridization (ISH) and GS-lectin immunohistochemistry (IHC). Representative images of dual RNAscope ISH (red) and GS-lectin IHC (B, C, E, F, H, I, endothelial cells, green) using mRNA probes for VEGF (A, C), HIF-1α (D, F) and HIF-2α (G, I). Nuclei were labelled with 4’,6-diamidino-2-phenylindole (DAPI) to distinguish the nuclear layers of the retina. All images were obtained using a 40× objective. Labels: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL).