Fig. 3.

Assessment of the cellular uptake of T4 procapsids containing packaged Alexa 488-labeled L-segment– and S-segment–derived ф6 dsDNA. (A and B) Histograms of the flow cytometry with varied concentrations of T4 procapsid-packaged Alexa 488-labeled L-segment (LL) and S-segment (SL) dsDNA, respectively. (C) The internalized Alexa 488-labeled M-segment (ML) dsDNA (∼2,000 bp, indicated by small arrows) within A549 cells was visualized by epifluorescent microscopy. (D) DAPI staining for nuclei (white arrow). (E) Overlay of C and D. (F) Bright-field image showing whole cells with nuclei indicated by the black arrow. (Scale bars, 10 µm.)