Table 1.
Sample receptor/ligand binding parameters
| Receptor | Ligand | Cell type | k− (1/s) | Kd (M) | τ (at L = Kd), s | Ref. |
| Fcε | IgE | Human basophils | 2.50E-05 | 4.80E-10 | 20,000.00 | (17) |
| Fcγ | 2.4G2 monoclonal Fab | Mouse macrophage | 3.80E-05 | 7.70E-10 | 13,157.89 | (18) |
| Canabinoid receptor | CP55,940 | Rat brain | 1.32E-04 | 2.10E-08 | 3,787.88 | (19) |
| IL-2 receptor | IL-2 | T cells | 2.00E-04 | 7.40E-12 | 2,500.00 | (20) |
| α1-Adrenergic | Prazosin | BC3H1 | 3.00E-04 | 7.50E-11 | 1,666.67 | (21) |
| Glucagon receptor | Glucagon | Rat hepatocytes | 4.30E-04 | 3.06E-10 | 1,162.79 | (22) |
| Formyl peptide receptor (FPR) | fMLP | Rat neutrophils | 5.50E-04 | 3.45E-08 | 909.09 | (23) |
| Ste2 (αF receptor) | αF | S. cerevisiae | 1.00E-03 | 5.50E-09 | 500.00 | (15, 16) |
| IFN | Human IFN-α1a | A549 | 1.20E-03 | 3.30E-10 | 416.67 | (24) |
| Transferrin | Transferrin | HepG2 | 1.70E-03 | 3.30E-08 | 294.12 | (25) |
| EGF receptor | EGF | Fetal rat lung | 2.00E-03 | 6.70E-10 | 250.00 | (26) |
| TNF | TNF | A549 | 2.30E-03 | 1.50E-10 | 217.39 | (24) |
| Insulin receptor | Insulin | Rat fat cells | 3.30E-03 | 2.10E-08 | 151.52 | (27) |
| FPR | FNLLP | Rabbit neutrophils | 6.70E-03 | 2.00E-08 | 74.63 | (28) |
| Total fibronectin receptors | Fibronectin | Fibroblasts | 1.00E-02 | 8.60E-07 | 50.00 | (29) |
| T-cell receptor | Class II MHC-peptide | 2B4 T-cells | 5.70E-02 | 6.00E-05 | 8.77 | (30) |
| FPR | N-formyl peptides | Human neutrophils | 1.70E-01 | 1.20E-07 | 2.94 | (31) |
| cAMP receptor | cAMP | D. discoideum | 1.00E+00 | 3.30E-09 | 0.50 | (32) |
| IL-5 receptor | IL-5 | COS | 1.47E+00 | 5.00E-09 | 0.34 | (33) |
| NMDA receptor | Glutamate | Hippocampal neurons | 5.00E+00 | 1.00E-06 | 0.10 | (34) |
| Adenosine A2A | Adenosine | HEK 293 (human) | 1.75E+01 | 5.20E-08 | 0.03 | (35) |
| AMPA receptor | Glutamate | HEK 293 (human) | 2.00E+03 | 5.00E-04 | 2.50E-04 | (36) |
A549, human lung alveolar carcinoma; BC3H1, smooth muscle-like cell line; COS, fibroblast-like cell line derived from monkey kidney tissue; 2.4G2 Fab, Fab portion of 2.4G2 antibody against receptor; fMLP, N-formyl-methionyl-leucyl-phenylalanine; FNLLP, N-formylnorleucylleucylphenylalanine; HepG2, human hepatoma cell line; τ, time it takes the binding reaction to reach 63% of its final (equilibrium) value. The value of τ depends on the concentration of the ligand (Fig. 1). Thus, we show the data for a concentration of ligand equal to the Kd of each reaction. Prazosin is an antagonist to the receptor.