Fig. 3.
Photosynthetic activity of mgdE mutant cells. (A) Oxygen evolution rates in wild-type (blue trace) and ΔmgdE (red trace) cells as a function of light intensity. Chl, chlorophyll. (B) Presence of the thylakoid membranes in ΔmgdE mutant cells. Ultrathin sections of wild-type (Left) and ΔmgdE mutant (Right) cells were examined by electron microscopy. Enlarged images of the boxed regions highlight the thylakoid membranes (arrowheads). (C) Pulse amplitude modulation fluorescence analysis of wild-type (black trace) and ΔmgdE (red trace) cells. Saturating pulses were applied during the actinic light irradiation (Act.). At the end of each measurement, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; 10 µM) was added to obtain the maximal fluorescence. (D) Comparison of parameters obtained in the pulse amplitude modulation fluorescence analysis for wild-type (blue bars) and ΔmgdE (orange bars) cells. Values presented are the average (wild-type, n = 15; ΔmgdE, n = 10) ± SE.