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. 2014 Sep 3;111(37):13469–13474. doi: 10.1073/pnas.1405414111

Fig. 1.

Fig. 1.

Expression patterns of amphioxus ALPs. (A and B) Expression of bjALP1 and bjALP2 mRNA in different tissues was determined by qRT-PCR analysis. The data are expressed as a ratio to the mRNA expression level of β-actin and are plotted as the means ± SD. (C) In situ hybridization of bjALP1 in adult female sections. (D) In situ hybridization of bjALP2 in adult female sections; the sense probe was used as the negative control. e, endostyle; g, gill; hc, hepatic cecum; i, intestine; m, muscle; nc, notochord; o, ovary; s, skin; sc, spinal cord. (E and F) The relative expression level of ALP mRNA in hepatic ceca and intestine after challenge with V. anguillarum and S. aureus was determined by qRT-PCR analysis. The data are shown as a ratio to the ALP mRNA expression level after the injection of PBS and are plotted as the means ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.