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. 2014 Sep 3;111(37):13469–13474. doi: 10.1073/pnas.1405414111

Fig. 3.

Fig. 3.

Amphioxus ALPs directly interacted with PGN. (A) ELISA analysis of the interaction between recombinant ALP proteins and microbial components. Plates were coated with 20-μg components then were washed and incubated with bjALPs at 4 °C overnight followed by detection with an anti-His antibody. One representative experiment of six is shown. Background absorbance without protein was subtracted. (B) Pull-down analysis of the binding of ALP proteins to PGN from S. aureus and E. coli. P, pellet protein; T, total protein. (C) ELISA analysis of the interaction between recombinant ALP proteins and MDP. One representative experiment of six is shown. Background absorbance without protein was subtracted. (D) ELISA analysis of the interaction between the truncated bjALP1 mutants and PGN. One representative experiment of six is shown. Background absorbance without protein was subtracted. *P < 0.05, **P < 0.01, ***P < 0.001.