Table 1. List of primary antibodies.
Common name, immunogen or clone, host species, dilution and source are indicated. Asterisks indicate antibodies already included in J Comp Neurol primary antibody's database.
| Polyclonal anti- | Immunogen, Host Species | Source, Catalog No. | Dilution, Titer (μg/ml) | |
|---|---|---|---|---|
| Iba1 (*) | Synthetic peptide corresponding to human Iba1 C-terminal amino acids 134-147, rabbit |
Wako (1), 019-19741 | 1:1,000 (0.5) | |
|
| ||||
| GFP | Full-length GFP (Aequorea victoria), goat | abcam (2), ab5450 | 1:2,000 (0.5) | |
|
| ||||
| Aquaporin-4 | Synthetic peptide corresponding to human AQP4 C-terminal amino acids 300-323, goat |
Santa Cruz (3), sc-9888 | 1:50 (4) | |
|
| ||||
| Brn3 (*) | Synthetic peptide corresponding to human Brn3b C-terminal amino acids 360-409, goat |
Santa Cruz (3), sc-6026 | 1:50 (4) | |
|
| ||||
| CRALBP (*) | Cellular retinaldehyde-binding protein isolated from bovine retina and RPE, rabbit |
John C. Saari (4) | 1:1,000 | |
|
| ||||
| Monoclonal anti- | Immunogen, Clone | Source, Catalog No. | Dilution | |
|
| ||||
| GFAP (*) | Porcine spinal cord GFAP, G-A-5 | Sigma (5), C9205 | 1:1,000 | |
| MAP1 | Rat brain MAPs, HM-1 | Sigma (5), M4278 | 1:300 | |
| Neurofilament | Human brain neurofilament, 2F11 | Dako (6), | M0762 1:50 | |
(*)
Antibodies listed in J Comp Neurol antibody's database.
1) Richmond, VA; 2) Cambridge, MA; 3) Santa Cruz, CA; 4) University of Washington, Seattle, WA; 5) Saint Louis, MO; 6) Carpinteria, CA.