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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunol. 2014 Aug 25;193(7):3409–3416. doi: 10.4049/jimmunol.1401194

Figure 2. Use of low TCR affinity peptide in peripheral tolerance protocol promotes CD8+ T cell deletion.

Figure 2

B10.D2 mice containing donor Thy1.1+ CL4 CD8+ T cells were treated daily with PBS, 10 μg of native Kd-HA or I512V peptide between days 0-2. (a) Day 3 flow cytometric analysis of donor CL4 cells in the spleen stained with antibody specific for the indicated molecule. Lines; Dashed - PBS, thin - native Kd-HA and thick – I512V treatments. (b) Day 3 ex vivo phosphorylation of Erk in CL4 cells re-stimulated with Kd-HA peptide (thin line) or unstimulated (dashed line). Treatment condition indicated in top right corner of plots and the mean MFI increase from unstimulated to stimulated cells in parenthesizes. (c) Survival of CL4 cells in the spleen. Expressed as the percentage of CL4 cells detected by FACS on day 6 compared to day 3 for each treatment condition. (d) On day 30 mice were challenge with PR8 influenza virus (I.P 500 HA units) and six days later donor CL4 cells were detected by FACS in the spleen. Each graph is representative of 2 to 3 experiments using age and sex matched mice.