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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunol. 2014 Aug 29;193(7):3816–3824. doi: 10.4049/jimmunol.1303256

Figure 2.

Figure 2

Leukocyte infiltration into single class II MHC-disparate renal allografts in CCR5-deficient and wild type C57BL/6 recipients. (A) bm12 renal allografts were harvested from groups of wild type C57BL/6 and B6.CCR5−/− recipients on the indicated days post-transplant. Following digestion of the allografts and bm12 kidneys from non-transplanted B6.H-2bm12 mice, aliquots of prepared single cells suspensions were stained with antibody and analyzed by flow cytometry to determine the numbers of graft infiltrating leukocyte populations, expressed as numbers/mg graft tissue ± SEM. *p < 0.05; **p < 0.01. Renal allografts from (B) C57BL/6 and (C) B6.CCR5−/− recipients were harvested on day 14 post-transplant and frozen sections were prepared and stained with hematoxylin and eosin or with anti-CD4 or anti-CD8 antibodies. Magnification ×400.