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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunol. 2014 Aug 29;193(7):3816–3824. doi: 10.4049/jimmunol.1303256

Figure 7.

Figure 7

Donor reactive CD8 T cells induced in response to single class II MHC-disparate bm12 renal allografts in B6.CCR5-deficient recipients react to I-Abm12 β chain peptides. Groups of wild type C57BL/6 and B6.CCR5-deficient mice received bm12 renal allografts. On day 14 post-transplant, CD8 T cells were purified from each of the recipient spleens and from the spleens of naïve mice and the frequency of CD8 T cells producing IFN-γ during culture with syngeneic C57Bl/6 or bm12 stimulator cells was quantified by ELISPOT assay. In the indicated cultures, 20 µg of the indicated control (SIINFEKL) or I-Abm12 β chain peptides were added at the initiation of the ELISPOT cultures. The results indicate the mean number of spots for splenic CD8 T cells from 4 individual mice per group ± SEM. p < 0.05 for all CCR5−/− recipients compared to wild type recipients with either bm12 or C57BL/6 plus bm12 peptide stimulator cells.