Figure 8.

Donor reactive CD8 T cells induced in response to single class II MHC-disparate bm12 cardiac allografts in B6.CCR5-deficient recipients react to cytokine-stimulated bm12 aortic endothelial cells. Groups of 4 wild type C57BL/6 and B6.CCR5-deficient mice received bm12 heart allografts. On day 10 post-transplant, CD8 T cells were purified from each of the recipient spleens and cultured with aortic endothelial cells from syngeneic C57BL/6 or bm12 mice that had been pre-activated with cytokines as detailed in the Materials and Methods. The activation of the CD8 T to produce IFN-γ after 24 hours of culture was quantified by ELISA of the culture supernatants. In the indicated cultures, 20 µg of K^b- or D^b-restricted peptides (#1 and #4 in Table 1) or control SIINFEKL peptide (S) were added at the initiation of the cultures. The results indicate the mean concentration of IFN-γ produced in CD8 T cell stimulation cultures from 4 individual allograft recipients per group ± SEM. *p < 0.05