TABLE 1.
New primers for qPCR: RNA expression analysis and genotyping
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| RNA analysis | ||
| Bcl11a | GTCTGCACACGGAGCTCTAA | CACTGGTGAATGGCTGTTTG |
| Bcl11b exons 2-4* | AGGTCACCCCTGATGAAGAT | CATGTGTGTTCTGTGCGTGCT |
| Bcl11b exon 4* | GGACAGCAATCCTTTCAACC | ATGACTCGGTCGAAGGCACT |
| CD44 | GCAGATCGATTTGAATGTAACC | GTCCGGGAGATACTGTAGC |
| Spi1 (PU.1) alternate | CTCAGTCACCAGGTTTCC | TCCAAGCCATCAGCTTCTC |
| Thy1 | GGGCGACTACTTTTGTGAGC | AGGGCTCCTGTTTCTCCTTG |
| TCRbC2 | GGGTTCTGTCTGCAACCATC | CTATGGCCAGGGTGAAGAAC |
| TCRg Cluster1 | GCTGTGAGCACTGTGATAGCTC | TCAGCAACAGAAGGAAGGAAA |
| TCR Vγ3(5)-Cγ1 | ATCGGATGAAGCCACGTACT | GAAGGAAAATAGTGGGCTTGG |
| TCR Vγ2(4)-Cγ1 | CGAAGCTATCTACTACTGTTCC | GAAGGAAAATAGTGGGCTTGG |
| TCRd | TACGACTGCTGTTTGCCAAG | TCTGAAGCACTGAGAAGTTGGA |
| Tcf7 (TCF-1) | CGAGAAGAGCAGGCCAAGTA | CCGAATGCATTTCTTTTTCC |
| Tcf7 alternate | TCCCCATGCCAATACTTCTT | GTTGGTGCCAAGGTTGAAAG |
| Zfpm1 (FOG1) alternate | CCAAGATGTCCGAGTTGGTG | GCGCTTGTGCACATAGAAGT |
| EBF-1 | CCATCCGAGTTCAGACACCT | ATGCCGAGGAATGACCTTCT |
| Pax5 E2/E3 | GAACTTGCCCATCAAGGTGT | GAGTGGCAACCTTTGGTTTG |
| Pax5 E5/E6 | GGGCTCCTCATACTCCATCA | CTGCTGCTGTGTGAACAGGT |
| Genotyping | ||
| GATA-3 Floxed | GGCATTCTCGCACGCTTCAAA (G3-F) | GGGCCGGTTCTGCCCATT (G3-R1) |
| GATA-3 Deleted | GGCATTCTCGCACGCTTCAAA (G3-F) | GGATGGGCACCACCCCGGTGAA (G3-R2) |
| Bcl11b Deleted | ACGCCGGACCTAGTAAATGCA (B-F) | GTTAGGCTGGACTGCCGCCTC (B-R) |