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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunol. 2014 Aug 29;193(7):3344–3354. doi: 10.4049/jimmunol.1400534

Figure 2. Notch signaling blockade results in reduced KIR expression.

Figure 2

KIR expression was evaluated on developing NK cells or adult NK cells after inhibition of the Notch pathway. (A–B) UCB progenitors were differentiated into NK cells by co-culture with EL08-1D2 stroma and cytokines for 21 days, harvested and cultured for an additional 7 days in 10 ng/ml IL-15 with DMSO or 20 uM gSI. Flow plots (A) and aggregate data (B) showing KIR expression on CD56+CD3− NK cells (n = 14). (C) Representative dot plot and (D) aggregate data of fresh sorted adult donor CD56+KIR− NK cells cultured for 7 days in 10 ng/ml IL-15 with DMSO or 20 uM gSI showing KIR expression on CD56+CD3− NK cells (n = 4). (E) Representative dot plot and (F) aggregate data of sorted CD56brightKIR− (top panels and open bars) or CD56dimKIR− (bottom panels and filled bars) NK cells cultured for 7 days in 10 ng/ml IL-15 with DMSO or 20 uM gSI showing KIR expression on CD56+CD3− NK cells (n = 5). On panel (F) CD56bright and CD56dim paired comparison were done separately as these represent different experiments.