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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunol. 2014 Aug 29;193(7):3344–3354. doi: 10.4049/jimmunol.1400534

Figure 6. Notch signaling can enhance NK cell function through CD16 crosslinking.

Figure 6

UCB CD34-derived NK cells (A–B, n = 15), sorted CD56brightKIR− PBNK cells (C–D, n = 7), or sorted CD56dimKIR− PBNK cells (E–F, n = 11) were cultured with 10 ng/ml IL-15 and OP9-N, OP9-DL1, or OP9-DL4 cells for 7 days. Cells were then cross-linked with (control) mouse anti-human IgG (open bars) or mouse anti-human CD16 (filled bars). (A, C, and E) CD107a expression on total CD56+CD3− NK cells or (B, D, and F) subpopulations based on KIR expression are shown. Repeated measures ANOVA with Tukey’s multiple comparison test was utilized for statistical analysis. For KIR break up panels separate tests were carried out for KIR+ and KIR− groups to diminish statistical clutter.