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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunol. 2014 Aug 20;193(7):3308–3321. doi: 10.4049/jimmunol.1400186

FIGURE 4. β-glucan, but not Pam3Cys, induced IL-10, IL-2 and IDO in vivo.

FIGURE 4

A) 10-week-old pre-diabetic female NOD mice (3/group) were injected with β-glucan and Pam3Cys for three alternate days or left untreated (control) as described in Fig. 3 and euthanized after 24 h. A) Spleen cells were cultured for 48h and supernatants were tested for spontaneously secreted cytokines by ELISA. B) cDNA prepared using spleen cells was subjected to real-time qPCR for detecting IDO. Expression levels were calculated relative to actin expression and the values of ligand-treated mice were compared against the value of untreated (none) group which was considered as 1. This experiment was repeated twice. Statistical significance of treated group was calculated against untreated (none) group. *, p<0.05; **, p <0.01; ***, p <0.001.